What type of bacteria does pea agar inhibit?

What type of bacteria does pea agar inhibit?

PEA agar is a selective medium that is used for the isolation of gram- positive Staphylococcus species andStreptococcus species from clinical specimens or specimens that contain mixtures of bacterial flora (2). Typically PEA agar is used to inhibit the common contaminants such as Escherichia coli and Proteus species.

How does pea agar inhibit gram negative bacteria?

Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram positive organisms. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces growth of Gram negative organisms by interfering with DNA synthesis. PEA also prevents Proteus species from swarming across the surface of the agar.

What grows on pea plate?

Staphylococcus aureus, a Gram-positive organism, grows on PEA while Serratia marcescens, a Gram-negative organism, does not.

What bacteria grows on Phenylethanol Agar?

Anaerobic PEA is used for the selective isolation of anaerobic bacteria from mixed populations and prevents rapidly-growing, facultatively anaerobic gram-negative bacteria (i.e. Proteus) from overgrowing strict anaerobes. The medium allows both gram-positive and most gram-negative obligately anaerobic bacteria to grow.

What does MacConkey agar test for?

MacConkey agar is used for the isolation of gram-negative enteric bacteria and the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria.

What type of media is pea?

Remel Phenylethyl Alcohol Agar (PEA) is a solid medium recommended for use in qualitative procedures for selective isolation of gram- positive cocci. Lilley and Brewer developed Phenylethyl Alcohol Agar for isolation of gram-positive cocci, such as staphylococci and streptococci, from specimens containing mixed flora.

What kind of bacteria can pea agar inhibit?

PEA should inhibit the growth of facultative anaerobic gram-negative rods, like Escherichia coli and swarming of Proteus mirabilis. PEA agar will not provide complete information for identification of bacterial isolates.

How big are colonies of E coli on blood agar?

Colonies of E.coli on blood agar 1 – 4 mm diameter after overnight incubation. Colonies may also appear mucoid and some strains of E.coli is hemolytic.

Can you grow Brucella blood agar on Pea?

Some organisms that would normally grow on PEA agar may be inhibited. It is recommended that a non-selective medium, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Some strains of facultative organisms (which should be inhibited) may grow on PEA.

What are the cultural characteristics of E coli?

Cultural Characteristics: 1 Blood Agar: Colonies of E.coli on blood agar 1 – 4 mm diameter after overnight incubation. 2 MacConkey agar: Escherichia coli ferment lactose. 3 CLED Agar: They produced yellow colonies on Cystine lactose electrolyte deficient (CLED) agar.

PEA should inhibit the growth of facultative anaerobic gram-negative rods, like Escherichia coli and swarming of Proteus mirabilis. PEA agar will not provide complete information for identification of bacterial isolates.

Which is the best atmosphere for pea Blood agar?

Depending on the objectives of the study, PEA blood agar plates can be incubated under aerobic, anaerobic, and 5% CO2 atmosphere. Incubation in a high CO2 atmosphere allows the detection of bacteria that require an increased CO2 concentration and also results in better growth of almost all of the other pathogens.

How is pea agar similar to Brucella blood agar?

Morphology of the colonies on PEA are similar to those found on enriched blood agar plates, like Brucella Blood Agar. PEA agar is especially useful in the selective isolation of anaerobes from mixed populations that contain rapidly growing gram-negative bacteria such as Proteus species.

How is phenylethyl alcohol used in pea agar?

PEA Product InsertPEA SDS. PEA agar has a nutritive base that consists of casein, soy peptone, meat peptone, yeast extract, dextrose, hemin, and vitamin K1. The selective agent, phenylethyl alcohol, reversibly inhibits DNA synthesis and thus inhibits facultative anaerobic gram-negative bacteria such as those of the family Enterobacteriaceae.