# What happens to total magnification when you switch from 4x to 10x?

## What happens to total magnification when you switch from 4x to 10x?

The total magnification of the microscope is equal to the magnification of the ocular multiplied by the magnification of the objective. If you are using the 4x objective and the 10x ocular the total magnification would be 4 x 10 = 40x.

**What happened to the size of the field of view as you switched from the 10x to the 40x objective?**

The field of view is inversely proportional to the magnification of the objective lens. For example, if the diameter of your field of view is 1.78 millimeters under 10x magnification, a 40x objective will be one-fourth as wide, or about 0.45 millimeters.

### What is the difference between 4x and 10x on a microscope?

For example, optical (light) microscopes are usually equipped with four objectives: 4x and 10x are low power objectives; 40x and 100õ are powerful ones. The total magnification (received with 10x eyepiece) of less than 400x characterizes the microscope as a low-powered model; more than 400x as a powerful one.

**What happens to the field size as you increase magnification?**

As magnification increases, the diameter of the field of view decreases. In other words, you can see less area of the specimen as you increase the magnification.

## What can you see at 40x magnification?

Microscope Magnification

- At 40x magnification you will be able to see 5mm.
- At 100x magnification you will be able to see 2mm.
- At 400x magnification you will be able to see 0.45mm, or 450 microns.
- At 1000x magnification you will be able to see 0.180mm, or 180 microns.
**What can you see at 400x magnification?**0.45mm

At 400x magnification you will be able to see 0.45mm, or 450 microns. At 1000x magnification you will be able to see 0.180mm, or 180 microns.### What are the 4 types of objective and their magnification?

What Are the Different Magnifications of Objective Lenses?

- Scanning Objective Lens (4x)
- Low Power Objective (10x)
- High Power Objective Lens (40x)
- Oil Immersion Objective Lens (100x)
- Specialty Objective Lenses (2x, 50x Oil, 60x and 100x Dry)

**What can you see at 100x magnification?**At 100x magnification you will be able to see 2mm. At 400x magnification you will be able to see 0.45mm, or 450 microns. At 1000x magnification you will be able to see 0.180mm, or 180 microns.

## What happens to the depth of field as total magnification decreases?

Decrease magnification? The less overall thickness you can see, so the depth of field is less. Lower the magnification, the greater the thickness you can see, so the greater the depth of field.

**How does the field of view change under a microscope?**D. Measurement of Object Size under the Microscope Note that as the magnifying power of the objective lens increasesthe diameter of the field of view decreases. Therefore, even though the field of view looks the same size to the viewer you are in fact looking at a smaller and smaller circle of light.

### How many hairs can fit across the high power field?

If three or four hairs could fit across the high power field: width (diameter) of each hair = 1/3 x 400 or 1/4 x 400 = 133 – 100 micrometers NOTE: In this example the diameter of the hair is closer to 100 micrometers (ie. approximately 4 hairs could fit acrossthe field of view) PAGE 16 ASSIGNMENT 1.

**How does magnification affect the depth of field?**The distance within which objects can be seen clearly (i.e. the depth of field) will get shorter and shorter as the magnification of the microscope is increased. Cells are viewed under high power so the fine focus must be adjusted to see a deeper cell or organelles within a cell (as with our cheek smear).

## How is the 4X objective used in a microscope?

4X– This objective magnifies the image by a factor of 4. It is referred to as the “scanning objective” since it is used to scan the slide to locate the specimen before viewing it at higher magnification. Your microscope may not have this objective lens, in which case you can begin with the 10X objective.

**How big is an objective field of view?**Data Table: Field of View. Objective Power Diameter (mm) Diameter ( µm) scanning power 4X 2.2 mm 2200 µm low power 10X .88 mm 880 µm High power 40X .22 mm 220 µm 1280 µm / 2.75 cells = ~465 µm as an average size of the onion cell.

D. Measurement of Object Size under the Microscope Note that as the magnifying power of the objective lens increasesthe diameter of the field of view decreases. Therefore, even though the field of view looks the same size to the viewer you are in fact looking at a smaller and smaller circle of light.

### What is the magnification of a 10x microscope?

10X– This objective magnifies the image by a factor of 10 and is referred to as the “low power” objective. 43X (or 45X)– This objective magnifies the image by a factor of 43 (or 45) and is referred to as the “high power” objective. 100X– This objective magnifies the image by a factor of 100.